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Santa Cruz Biotechnology anti matrix gla protein
Fig. 2. Effect of confluence on vascular smooth muscle cells (VSMCs). A: effect on total sodium-dependent and sodium-independent inorganic phosphate (Pi) uptakes as a function of tissue culture confluence. Symbols are the mean of 3 experimental values. B: expression of the indicated RNAs in VSMCs at 50% (black bars) and 100% (white bars) confluence. Bars show the means of 3 data. C: protein expression of the corresponding RNAs analyzed in B and expressed in relation to -actin. Representative Western blots of target proteins are shown at the right. When more than one band is present, the specific signal is shown with an arrowhead. All Western blots were normalized to -actin, which was unchanged between samples on all blots showing no errors in loading. Full-length images of all target genes and -actin for all blots were provided during peer review. Only representative blots of target genes are shown for brevity. PiT1 and PiT2, inorganic phosphate transporters 1 and 2; MGP, matrix Gla protein; -SMA, -smooth muscle actin. *P  0.05, mean significant differences with a t-test in B and C. Bars are the means of 3–6 values.
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Proteintech matrix gla protein rp 2 n a proteintech
Fig. 2. Effect of confluence on vascular smooth muscle cells (VSMCs). A: effect on total sodium-dependent and sodium-independent inorganic phosphate (Pi) uptakes as a function of tissue culture confluence. Symbols are the mean of 3 experimental values. B: expression of the indicated RNAs in VSMCs at 50% (black bars) and 100% (white bars) confluence. Bars show the means of 3 data. C: protein expression of the corresponding RNAs analyzed in B and expressed in relation to -actin. Representative Western blots of target proteins are shown at the right. When more than one band is present, the specific signal is shown with an arrowhead. All Western blots were normalized to -actin, which was unchanged between samples on all blots showing no errors in loading. Full-length images of all target genes and -actin for all blots were provided during peer review. Only representative blots of target genes are shown for brevity. PiT1 and PiT2, inorganic phosphate transporters 1 and 2; MGP, matrix Gla protein; -SMA, -smooth muscle actin. *P  0.05, mean significant differences with a t-test in B and C. Bars are the means of 3–6 values.
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Matrix Gla Protein MGP Antibody FITC is an antibody conjugated to FITC against Matrix Gla Protein MGP
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Rabbit anti-Human MGP Polyclonal Antibody
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The MGP Antibody from Novus is a MGP antibody to MGP. This antibody reacts with Human. The MGP antibody has been validated for the following applications: Immunohistochemistry, Immunohistochemistry-Paraffin.
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Image Search Results


Fig. 2. Effect of confluence on vascular smooth muscle cells (VSMCs). A: effect on total sodium-dependent and sodium-independent inorganic phosphate (Pi) uptakes as a function of tissue culture confluence. Symbols are the mean of 3 experimental values. B: expression of the indicated RNAs in VSMCs at 50% (black bars) and 100% (white bars) confluence. Bars show the means of 3 data. C: protein expression of the corresponding RNAs analyzed in B and expressed in relation to -actin. Representative Western blots of target proteins are shown at the right. When more than one band is present, the specific signal is shown with an arrowhead. All Western blots were normalized to -actin, which was unchanged between samples on all blots showing no errors in loading. Full-length images of all target genes and -actin for all blots were provided during peer review. Only representative blots of target genes are shown for brevity. PiT1 and PiT2, inorganic phosphate transporters 1 and 2; MGP, matrix Gla protein; -SMA, -smooth muscle actin. *P  0.05, mean significant differences with a t-test in B and C. Bars are the means of 3–6 values.

Journal: American journal of physiology. Heart and circulatory physiology

Article Title: Several phosphate transport processes are present in vascular smooth muscle cells.

doi: 10.1152/ajpheart.00433.2019

Figure Lengend Snippet: Fig. 2. Effect of confluence on vascular smooth muscle cells (VSMCs). A: effect on total sodium-dependent and sodium-independent inorganic phosphate (Pi) uptakes as a function of tissue culture confluence. Symbols are the mean of 3 experimental values. B: expression of the indicated RNAs in VSMCs at 50% (black bars) and 100% (white bars) confluence. Bars show the means of 3 data. C: protein expression of the corresponding RNAs analyzed in B and expressed in relation to -actin. Representative Western blots of target proteins are shown at the right. When more than one band is present, the specific signal is shown with an arrowhead. All Western blots were normalized to -actin, which was unchanged between samples on all blots showing no errors in loading. Full-length images of all target genes and -actin for all blots were provided during peer review. Only representative blots of target genes are shown for brevity. PiT1 and PiT2, inorganic phosphate transporters 1 and 2; MGP, matrix Gla protein; -SMA, -smooth muscle actin. *P 0.05, mean significant differences with a t-test in B and C. Bars are the means of 3–6 values.

Article Snippet: Western blot analyses were performed as described (9) with the following commercial antibodies: anti-calponin 1 (sc-16604-R), anti-matrix Gla protein (anti-MGP; sc-66965), and anti-Sm22 (sc-18513), which were all from Santa Cruz Biotechnology (Heidelberg, Germany); anti- -smooth muscle actin (A5228) and anti- -actin (A1978) antibodies were from Sigma Aldrich; and anti-PiT1 and anti-PiT2 were polyclonal antibodies reported previously (9).

Techniques: Expressing, Western Blot